International Journal of Plant Protection

H. J. Kapadiya ¹, D. M Pathak ², D. R. Patel ²

Affiliations
1 College of Agriculture, Junagadh Agricultural University, Amereli Gujarat, IN
2 College of Agriculture, Navsari Agricultural University, Bharuch Gujarat, IN

Abstract

Experiment was conducted to determine effect of artificial injuries and fresh neck cutting to onion bulb on incidence of black mould disease. Onion bulbs were artificially injured at different sites viz., top, side, bottom, scattered and fresh neck cutting. Injured and un injured onion bulbs were inoculated with spore suspension (1x106 /ml) of Aspergillus niger. Without dipping in conidial suspension were served as check. The selected bulbs were packed in sterilized polythene bags and stored at room temperature for 21 days. Maximum black mould disease intensity (38.86 %) and weight loss (5.44 %) was recorded in the fresh neck cutting followed by top, scattered, middle and bottom injuries. The check treatment recorded minimum black mould (10.81 %) intensity and weight loss (2.66 %).

Keywords

Onion Artificial Injuries, Neck Cutting, Aspergillus Niger, Black Mould, Post Harvest

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M. J. Sardhara ¹, D. K. Davara ², A. M. Moradia ², H. J. Kapadiya ³

Affiliations
1 Department of Plant Pathology, Junagadh Agricultural University, Junagadh (Karnataka), IN
2 Main Oilseeds Research Station, Junagadh Agricultural University, Junagadh (Karnataka), IN
3 Main Oilseeds Research Station, Junagadh Agricultural University, Junagadh (Karnataka), IN

Abstract

Anthracnose (Colletotrichum lindemuthianum) fungus is an important in the urdbean legumes crop. In the present investigation, the rate of growth of Anthracnose (C. lindemuthianum) has been compared in various temperature, culture media solid and liquid while in case solid media types viz., simple PDA (Potato Dextrose Agar), Potato carrot sucrose agar, Carrot juice agar, Blackgram leaf extract agar, Richard's agar, Czapeck's (Dox) agar, Asthana and Hawker's agar had tested. Among all the solid media tested, maximum mycelial growth was obtained in Potato dextrose agar medium (80.04 mm), which was statistically at par with Richard's agar medium (76.52 mm). Similarly in the liquid media, maximum dry mycelial weight was recorded in Richard's broth medium (444.34 mg) after 10 days of inoculation which was significantly superior over rest of the broth medias and followed potato dextrose agar broth (429.03 mg). Temperature plays an important role in infection and disease development. Maximum mean colony diameter of fungus was recorded at temperatures of 30°C (80.92 mm) and 25°C (78.62 mm) which was significantly superior over all other temperatures. Lowest mean colony diameter was obtained at temperatures of 10° (24.01 mm) and 40°C (21.37 mm). This is important for further study of disease management.

Keywords

Blackgram (Vigna mungo L. Hepper), Culture Media, Temperature.

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